Electrophoretic mobility shift assays implicate XRCC2:rs3218550C>T as a potential low-penetrant susceptibility allele for sporadic breast cancer

Electrophoretic mobility shift assays for RNA-protein complexes.

The electrophoretic mobility shift assay (EMSA), or gel mobility shift assay, is a popular and powerful technique for the detection of RNA-protein interactions. It relies on the fact that naked RNA has certain mobility on nondenaturing gels, but if the RNA is bound by protein, the mobility of the RNA is reduced. Therefore, the binding of protein results in a characteristic upward shift of the R...

The potential of electrophoretic mobility shift assays for clinical mutation detection.

As the understanding of the links between genetic mutations and diseases continues to grow, there is an increasing need for techniques that can rapidly, inexpensively, and sensitively detect DNA sequence alterations. Typically, such analyses are performed on PCR-amplified gene regions. Automated DNA sequencing by capillary array electrophoresis can be used, but is expensive to apply to large nu...

Ku autoantigen is a potential major cause of nonspecific bands in electrophoretic mobility shift assays.

Electrophoretic Mobility Shift Assay (EMSA).

Transcriptional regulation of gene expression is controlled through the binding of sequence-specific DNA-binding proteins (transcription factors) to the regulatory regions of genes. The exact gene expression program of a cell is determined by the spectrum of transcription factors present with the nucleus of a cell. The presence of these factors is dependent upon the cell type being examined and...

Use of fluorescently labeled DNA and a scanner for electrophoretic mobility shift assays.

thaliana plants. C. R. Acad. Sci. (Paris) 316:1194-1199. 2.Devic, M., S. Albert, M. Delseny, and T.J. Roscoe. 1997. Efficient PCR walking on plant genomic DNA. Plant Physiol. Biochem. 35:331-339. 3.Doyle, J.J. and J. L Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. 4.Frey, M., C. Stettner, and A. Gierl. 1998. General method for gene isolation in tagging approaches: Ampli...